How cytometer can Save You Time, Stress, and Money.
How cytometer can Save You Time, Stress, and Money.
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Information-enriched characterization of mobile populations. Morphological information and facts from visuals can add into the richness of flow cytometry knowledge. For instance, the figure demonstrates an or else typical apoptosis assay working with Annexin V and PI, introducing mobile imaging to characterize cells in Every population to reveal morphologically distinct capabilities. These insights couldn't are already received from multiplex staining by itself.
As samples are acquired about the Attune CytPix Flow Cytometer, the large-speed brightfield camera captures and stores images of detected occasions in a rate approximately 6,000 photos for each second, based on the flow level and picture sizing. For bigger overall flexibility, Attune Cytometric Software program enables you to adjust the picture capture frequency as essential and select a certain gate for graphic seize, if necessary.
Merge acoustic focusing technologies with high-parameter Assessment for versatile spectral unmixing and traditional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating which has a higher-velocity brightfield digital camera for simultaneous large throughput flow cytometry and superior resolution brightfield imaging.
"An image is not pretty worth a thousand dots, however it truly helps you to see visuals. It is great to view photos that correlate with staining. Such as, AnnexinV-constructive cells that happen to be morphologically various than balanced cells, or CD14+ monocytes that are much larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, a highly skilled consumer has gated singlets confidently. Soon after evaluating the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate has a lot more than 4% aggregates.
Pair your Attune flow cytometer with an autosampler for increased workflow efficiency, and insert robotic integration for max throughput.
Consistent image high-quality even at large throughput Acoustic concentrating and also a higher-speed camera Blend to picture these T cells regularly at reduced or significant flow rates. Simply change concentrate and digital camera settings to meet experimental needs.
Early signs of this contamination had originally been dismissed as debris. Backgating to discover new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also helps you to use morphological attributes to find appealing subpopulations that would not be obvious from flow cytometry details by yourself.
Maybe most importantly, these gatherings include cells of Obviously different phenotypes which may lead to incorrect conclusions regarding double constructive events (especially in scarce populations).
AI-driven automatic graphic Investigation with the Attune Cytometric Software package translates celebration capabilities into unique morphology parameters that can be coupled with regular fluorescence and scatter parameters to expose highly reproducible population modifications and special subsets.
Application Notice: Automatic impression Assessment lessens consumer-to-person variability in flow cytometry gating techniques
Developed-in dimensionality reduction equipment enhanced resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to substantiate check here envisioned populace resolution in lysed total human blood. User can check out occasions from Every single cluster for visual confirmation of mobile phenotypes.
No wash/no lyse leukocyte Investigation with imaging. Cells have been acquired from 24-hour-outdated blood by dilution in one mM EDTA ( Qualified UX/UI permits consumers to educate AI versions for expanded apps. (A) The “Train Design” attribute supplies annotation equipment and true-time model coaching precision.
At the heart of each and every Attune Flow Cytometer lies our innovative acoustic focusing technological innovation, which utilizes seem waves to meticulously align cells within the flow cell. This impressive strategy surpasses regular hydrodynamic procedures, leading to more quickly processing speeds, Excellent knowledge excellent, and amplified sample throughput.
The Attune Xenith cytometer is really a high-parameter spectral analyzer, furnishing both of those spectral unmixing and standard flow capabilities for in depth and adaptable cell Evaluation.
Agilent Value Promise assures you at the least 10 years of instrument use from your day you buy. Normally, We're going to credit rating you With all the residual value of the process towards an upgraded model.
What gave the impression to be a homogeneous populace is in fact additional assorted—an Perception that ought to be viewed as when interpreting outcomes.